Trapping folding intermediates
نویسندگان
چکیده
منابع مشابه
Characterizing protein folding intermediates.
To determine the pathway by which a protein folds up, it is necessary to characterize the structures of folding intermediates and also to place these intermediates in the correct order on the kinetic pathway of folding. Noncovalent folding reactions are fast: typically they occur in seconds or less for small single-domain proteins. On the other hand, hours are required to obtain detailed struct...
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Our recent experiments on the molten globule state and other protein folding intermediates lead to following conclusions: (i) the molten globule is separated by intramolecular first-order phase transitions from the native and unfolded states and therefore is a specific thermodynamic state of protein molecules; (ii) the novel equilibrium folding intermediate (the 'pre-molten globule' state) exis...
متن کاملKinetic and equilibrium folding intermediates
O u r recent experim ents on the m olten globule state and o ther protein folding in term ediates lead to following conclusions: (i) the m olten globule is separated by in tram olecular first-order phase transitions from the native and unfolded states and therefore is a specific therm odynam ic state of protein molecules; (ii) the novel equilibrium folding in term ediate (the ‘pre-m olten g lob...
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Cytochrome-c (cyt-c) is an electron transport protein, and it is present throughout the evolution. More than 280 sequences have been reported in the protein sequence database (www.uniprot.org). Though sequentially diverse, cyt-c has essentially retained its tertiary structure or fold. Thus a vast data set of varied sequences with retention of similar structure and fun...
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The oxidation of three different complexes of the doubly cycloplatinated 2,6-di(4-fluorophenyl)pyridine ligand (namely DMSO, PPh3 and PPr3 derivatives, 1a, 1b and 1c, respectively) with the electrophilic oxidant iodobenzenedichloride was studied. In each case oxidation can yield a simple trans-dichloro platinum(iv) complex (2(t)), which subsequently isomerises to the cis isomer (2(c)). However,...
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ژورنال
عنوان ژورنال: Nature
سال: 1988
ISSN: 0028-0836,1476-4687
DOI: 10.1038/335666a0